纤细裸藻中的甲羟戊酸激酶
Mevalonic acid kinase in Euglena gracilis.
作者信息
Cooper C Z, Benedict C R
出版信息
Plant Physiol. 1967 Apr;42(4):515-9. doi: 10.1104/pp.42.4.515.
Abstract
The isolation and partial purification of mevalonic acid kinase from Euglena gracilis is described. The product of the reaction MVA-5-P has been characterized by paper chromatography. The apparent Km values for l-mevalonic acid, ATP, and Mg(2+) are 3 x 10(-5)m, 6 x 10(-3)m, and 9 x 10(-3)m, respectively. A concentration of 1 x 10(-3)mp-hydroxymercuribenzoate completely inactivates the enzyme. A distribution study has shown that mevalonic acid kinase is present in most higher plants and the algae Euglena gracilis and Chlamydomonas. No enzymatic activity could be detected in several species of photosynthetic bacteria or blue-green algae.
摘要
本文描述了从纤细裸藻中分离和部分纯化甲羟戊酸激酶的过程。反应产物MVA-5-P已通过纸色谱法进行了表征。L-甲羟戊酸、ATP和Mg(2+)的表观Km值分别为3×10(-5)m、6×10(-3)m和9×10(-3)m。浓度为1×10(-3)m的对羟基汞苯甲酸可使该酶完全失活。一项分布研究表明，甲羟戊酸激酶存在于大多数高等植物以及藻类纤细裸藻和衣藻中。在几种光合细菌或蓝藻中未检测到酶活性。
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