Drobis J D, Siraganian R P
J Immunol. 1976 Sep;117(3):1049-53.
Human peripheral basophils can be maintained in cultured for up to 72 hr. These cells retain their functional integrity as judged by total histamine content and by their ability to release histamine by an IgE-mediated reaction in response to a specific antigen challenge. Cells cultured after suboptimal antigenic challenge could be activated to release histamine upon the addition of antigen. In contrast, cells culture after supra-optimal antigenic challenge did not fully recover their ability to release histamine even after 24 hr. With a variety of culture conditions, it was impossible to demonstrate any net synthesis of histamine in cells. Cells cultured after depletion of their stores by reaction with antigen did not show any net histamine formation. The experiments suggest that the basophil in peripheral blood is functionally an end-stage cell and can participate in the histamine release reaction only once.
人外周血嗜碱性粒细胞可在培养中维持长达72小时。根据总组胺含量以及它们在IgE介导的反应中通过特定抗原刺激释放组胺的能力判断，这些细胞保持其功能完整性。次优抗原刺激后培养的细胞在添加抗原时可被激活释放组胺。相比之下，超优抗原刺激后培养的细胞即使在24小时后也未完全恢复其释放组胺的能力。在各种培养条件下，均无法证明细胞中有组胺的任何净合成。与抗原反应耗尽其储存后培养的细胞未显示任何组胺净形成。这些实验表明，外周血中的嗜碱性粒细胞在功能上是终末阶段细胞，并且只能参与一次组胺释放反应。
