Winterbourne D J, Mora P T
J Supramol Struct. 1977;7(1):91-100. doi: 10.1002/jss.400070109.
Analysis of glucosamine labeled glycoconjugates in cultured cells has been made comparing 2 clones and the parent embryonic mouse cell line. Hyaluronic acid, heparan sulphate, and chondrotin sulphate as well as a complex mixture of glycopeptides were found in the medium, the trypsinate, and the trypsinized cells, although the distribution was not uniform. The 3 cell lines had very similar in vitro growth properties, including their plating efficiency in viscous medium. However, the tumorigenicity of the cells, determined syngeneic mice, was found to differ. All 3 cell lines were found to have similar glycoconjugate distributions, although a slight relative increase in labeled hyaluronic acid was found in the more tumorigenic mass cell line than either of the clones. The possible significance of this increase is discussed.
已对培养细胞中氨基葡萄糖标记的糖缀合物进行分析，比较了两个克隆和原始胚胎小鼠细胞系。在培养基、胰蛋白酶消化物和胰蛋白酶处理的细胞中发现了透明质酸、硫酸乙酰肝素、硫酸软骨素以及糖肽的复杂混合物，尽管分布并不均匀。这三种细胞系具有非常相似的体外生长特性，包括它们在粘性培养基中的接种效率。然而，通过同基因小鼠确定的细胞致瘤性存在差异。尽管在致瘤性更强的大量细胞系中发现标记的透明质酸相对于两个克隆中的任何一个都略有相对增加，但发现所有三种细胞系具有相似的糖缀合物分布。讨论了这种增加可能的意义。
