Miller E R, Harms R H, Wilson H R
Poult Sci. 1977 Mar;56(2):586-9. doi: 10.3382/ps.0560586.
Three trials were conducted to measure changes in the serum phosphorus level of laying hens over a 24-hour period. Sixty-three-week-old hens were selected for blood sampling according to the criteria that: 1. they laid an egg the day prior to and the day of blood collection and 2. the oviposition time on both days was 10 a.m. +/- 2 hours. Blood samples were obtained via heart puncture. Serum was collected by centrifugation, deproteinized and analyzed colorimetrically for phosphorus content. Sampling times were as follows: Trial 1--6 p.m., 12 midnight, 6 a.m. and 12 noon; Trial 2--6 a.m., 8 a.m., 10 a.m., 12 noon, 2 p.m., 4 p.m. and 6 p.m.; Trial 3--12 midnight, 4 a.m. and 7 a.m. Hens used in this study received natural and/or artifical light from 4:30 a.m. to 7:30 p.m. each day. Results indicated that serum phosphorus reached a peak of 6.08 mg.% at 7 a.m. At 8 a.m., the phosphorus level dropped to 4.88 mg.% and remained within 0.08 mg.% of this value until 12 noon. From 2 p.m. to 6 a.m. it gradually increased from 5.29 mg9% to 5.80 mg.%. Observed changes in serum phosphorus over time are related to changes in shell calcification, bone resorption and remineralization, and renal clearance.
进行了三项试验以测量产蛋母鸡在24小时内血清磷水平的变化。根据以下标准选择63周龄的母鸡进行采血：1. 在采血前一天和采血当天均产蛋；2. 这两天的产蛋时间均为上午10点正负2小时。通过心脏穿刺获取血样。通过离心收集血清，进行脱蛋白处理，并采用比色法分析磷含量。采样时间如下：试验1——下午6点、午夜12点、上午6点和中午12点；试验2——上午6点、上午8点、上午10点、中午12点、下午2点、下午4点和下午6点；试验3——午夜12点、凌晨4点和上午7点。本研究中使用的母鸡每天从凌晨4:30至晚上7:30接受自然光和/或人工光照。结果表明，血清磷在上午7点达到峰值6.08mg.%。上午8点，磷水平降至4.88mg.%，并在中午12点前一直保持在该值的0.08mg.%范围内。从下午2点至凌晨6点，它从5.29mg.%逐渐增加到5.80mg.%。观察到的血清磷随时间的变化与蛋壳钙化、骨吸收和再矿化以及肾脏清除的变化有关。
