Sarvella P, Roberts D, Morris R
Poult Sci. 1977 Jul;56(4):1269-72. doi: 10.3382/ps.0561269.
Sera from chicken, turkey, pheasant, quail and their intergeneric hybrids were compared by their reactions in two-dimensional immunoelectrophorsis with homologous and heterologous antisera. As many as 44 antigenically distinguishable components were resolved in these sera by use of this technique. Sera from the intergeneric hybrids, when allowed to react with rabbit anti-White Leghorn antiserum, gave more precipitin peaks than did any of the parental sera in reaction with this same antiserum. Thus it appeared that intergeneric hybridization induced a propagation of avian serum antigens recognized by rabbit antiserum to a single parental serum. For example, all parental sera, in reaction with homologous or heterologous antisera, appeared to contain only one precipitin peak which migrated in the albumin area. Sera from the intergeneric hybrids of chicken X turkey, chicken X pheasant, and chicken X quail had two antigenetically distinguishable peaks in the albumin area. Sera from turkey X pheasant and pheasant X quail had only one peak in this region. These results agree with the observations obtained from polyacrylamide disc gel electrophoresis of the plasma proteins.
通过二维免疫电泳中与同源和异源抗血清的反应，对鸡、火鸡、雉鸡、鹌鹑及其属间杂种的血清进行了比较。利用该技术，在这些血清中分辨出了多达44种抗原性可区分的成分。属间杂种的血清与兔抗白来航鸡抗血清反应时，与任何一种亲本血清与同一种抗血清反应相比，产生的沉淀素峰更多。因此，似乎属间杂交诱导了兔抗血清所识别的禽类血清抗原向单一亲本血清的增殖。例如，所有亲本血清与同源或异源抗血清反应时，似乎只含有一个在白蛋白区域迁移的沉淀素峰。鸡×火鸡、鸡×雉鸡和鸡×鹌鹑的属间杂种血清在白蛋白区域有两个抗原性可区分的峰。火鸡×雉鸡和雉鸡×鹌鹑的血清在该区域只有一个峰。这些结果与从血浆蛋白的聚丙烯酰胺圆盘凝胶电泳中获得观察结果一致。
