真核细胞中受损DNA的修复：梨形四膜虫

Repair of damaged DNA in a eucaryotic cell: Tetrahymena pyriformis.

作者信息

Brunk C F, Hanawalt P C

出版信息

Science. 1967 Nov 3;158(3801):663-4. doi: 10.1126/science.158.3801.663.

DOI:10.1126/science.158.3801.663

Abstract

Damage induced by ultraviolet light or x-rays to the DNA of a eucaryotic organism, Tetrahymena pyriformis, is repaired by a process similar to the repair system present in bacteria. This repair process, which involves defect excision and subsequent resynthesis of the damaged section of DNA, occurs in the dark. Photoreactivation of damage induced by ultraviolet light is also indicated by a reduction in observed repair synthesis. An improved method for detecting repair synthesis is described. Repair synthesis is measured in parental DNA strands isolated from cultures that have undergone normal DNA replication after the repair process.

摘要

紫外线或X射线对真核生物梨形四膜虫的DNA造成的损伤，可通过一种类似于细菌中存在的修复系统的过程进行修复。这个修复过程涉及缺陷切除以及随后受损DNA片段的重新合成，它在黑暗中发生。紫外线诱导损伤的光复活也通过观察到的修复合成减少来表明。本文描述了一种改进的检测修复合成的方法。修复合成是在从经过修复过程后进行正常DNA复制的培养物中分离出的亲本DNA链中进行测量的。