嗜热四膜虫基因启动子区域的特征分析
Characterization of the promoter region of Tetrahymena genes.
作者信息
Brunk C F, Sadler L A
机构信息
Biology Department, University of California, Los Angeles 90024-1606.
出版信息
Nucleic Acids Res. 1990 Jan 25;18(2):323-9. doi: 10.1093/nar/18.2.323.
Abstract
The regions between adjacent histone H3 and H4 genes, as well as portions of the genes, from 22 species of Tetrahymena have been amplified using the polymerase chain reaction and sequenced. Both histone genes are transcribed divergently with initiation occurring within the intergenic region, thus 2 sets of 22 homologous Tetrahymena promoters can be compared. A sequence comparison of these regions reveals a single putative promoter element, with a consensus sequence TATCCAATTCARA, present in front of each gene. This sequence contains a 'CCAAT' box, which also occurs at 8 locations preceding other ciliate genes. No other putative promoter sequences are found in front of these sets of histone genes. Sequences searched for include 'TATA' boxes, 'GC' boxes and other sequences suggested as putative promoter elements for ciliate genes. The coding strand immediately preceding ciliate genes is very high in A content and the consensus sequence at the site of protein synthesis is AAAATGG.
摘要
利用聚合酶链反应扩增并测序了来自22种四膜虫的相邻组蛋白H3和H4基因之间的区域以及部分基因。两个组蛋白基因以发散方式转录，起始发生在基因间区域内，因此可以比较22组同源的四膜虫启动子。对这些区域的序列比较揭示了一个单一的假定启动子元件，其共有序列为TATCCAATTCARA，存在于每个基因之前。该序列包含一个“CCAAT”框，它也出现在其他纤毛虫基因之前的8个位置。在这些组蛋白基因之前未发现其他假定的启动子序列。搜索的序列包括“TATA”框、“GC”框以及其他被认为是纤毛虫基因假定启动子元件的序列。紧接纤毛虫基因之前的编码链的A含量非常高，蛋白质合成位点的共有序列为AAAATGG。
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