Tateyama S, Cotchin E
Res Vet Sci. 1977 Nov;23(3):356-64.
Alkaline phosphatase (ALK-P) reactions at the light and electron microscopic levels were performed on eight canine mammary mixed tumours. In the morphologically normal gland next to the tumours, the myoepithelial cells and the stromal tissues near the acinar basement membranes both showed a positive ALK-P reaction by light microscopy. At the electron microscopic level, those parts of the myoepithelial cell membrane that were in contact with other cells--myoepithelial or lumenal epithelial--showed an ALK-P-positive reaction, as did the adjacent stromal tissue. The characteristic tumour cells, at sites of early proliferation, were ALK-P-positive, while in the mucoid and chondroid areas of the mixed tumours they were largely ALK-P-negative by light microscopy. By electron microscopy, those neoplastic cells which were in contact with other cells typically showed a positive ALK-P reaction, while those cells which were in contact with mucoid or chondroid matrix were largely negative. Although the cytoplasmic filaments of the neoplastic cells were 10 nm thick, and those of normal myoepithelial cells were 6 to 8 nm thick, the observations reported provide further evidence for the view that the essential cells of the canine mammary mixed tumour are derived from myoepithelial cells.
对8例犬乳腺混合瘤进行了光镜和电镜水平的碱性磷酸酶（ALK-P）反应。在肿瘤旁形态学正常的腺体中，肌上皮细胞和腺泡基底膜附近的间质组织在光镜下均显示ALK-P阳性反应。在电镜水平上，肌上皮细胞膜与其他细胞（肌上皮细胞或管腔上皮细胞）接触的部分以及相邻的间质组织均显示ALK-P阳性反应。在早期增殖部位的特征性肿瘤细胞为ALK-P阳性，而在混合瘤的黏液样和软骨样区域，光镜下它们大多为ALK-P阴性。通过电镜观察，与其他细胞接触的肿瘤细胞通常显示ALK-P阳性反应，而与黏液样或软骨样基质接触的细胞大多为阴性。尽管肿瘤细胞的细胞质细丝直径为10 nm，正常肌上皮细胞的为6至8 nm，但所报道的观察结果进一步证明了犬乳腺混合瘤的基本细胞来源于肌上皮细胞这一观点。
