肾上腺皮质胆固醇侧链裂解酶系统的特异性

Specificity of the cholesterol side-chain cleavage enzyme system of adrenal cortex.

作者信息

Burstein S, Nicholson R C, Byon C Y, Kimball H L, Gut M

出版信息

Steroids. 1977 Oct;30(4):439-53. doi: 10.1016/0039-128x(77)90092-7.

Abstract

Incubation of lanosta-8, 24-dien-3beta-o1-1,2-3H and lanost-8-en-3beta-o1-1,2-3H with an adrenocortical bovine mitochondrial acetone-dried preparation did not yield any significant (less than 0.01%) 3beta-hydroxy-4, 4, 14-trimethyl-5alpha-pregn-8-en-20-one. Under the same conditions cholesterol-1,2-3H yielded 8.3% pregnenolone. Incubation of (20S)-17alpha, 20-di-hydroxycholesterol-7-3H yielded 0.6 to 1.6% (20S,22R)-17alpha, 20, 22-trihydroxycholesterol, 1.0 to 3.2% of 17alpha-hydroxy-pregnenolone, but no significant (less than 0.02%) (20S,22S)-17alpha,20,22-trihydroxycholesterol. In another experiment incubation of cholesterol-1,2-3H yielded 5% pregnenolone, 0.5% 17alpha-hydroxypregnenolone, 0.2% (20R,22R)-20,22-dihydroxy-cholesterol, but no significant ( less than 0.01%) 17alpha-hydroxy-cholesterol, (20S)-17alpha, 20-dihydroxycholesterol or (20S,22R)-17alpha, 20,22-trihydroxycholesterol.

摘要

将羊毛甾-8,24-二烯-3β-醇-1,2-³H和羊毛甾-8-烯-3β-醇-1,2-³H与肾上腺皮质牛线粒体丙酮干燥制剂一起温育，未产生任何显著（小于0.01%）的3β-羟基-4,4,14-三甲基-5α-孕甾-8-烯-20-酮。在相同条件下，胆固醇-1,2-³H产生了8.3%的孕烯醇酮。将(20S)-17α,20-二羟基胆固醇-7-³H温育，产生了0.6%至1.6%的(20S,22R)-17α,20,22-三羟基胆固醇、1.0%至3.2%的17α-羟基孕烯醇酮，但未产生显著（小于0.02%）的(20S,22S)-17α,20,22-三羟基胆固醇。在另一项实验中，胆固醇-1,2-³H温育产生了5%的孕烯醇酮、0.5%的17α-羟基孕烯醇酮、0.2%的(20R,22R)-20,22-二羟基胆固醇，但未产生显著（小于0.01%）的17α-羟基胆固醇、(20S)-17α,20-二羟基胆固醇或(20S,22R)-17α,20,22-三羟基胆固醇。