灌注兔肝中胆汁醇的代谢 - C26胆汁醇

Metabolism of bile alcohols in the perfused rabbit liver - C26 bile alcohols.

作者信息

Cohen B I, Kuramoto T, Rothschild M A, Graham R, Mosbach E H

出版信息

Steroids. 1977 Oct;30(4):541-59. doi: 10.1016/0039-128x(77)90099-x.

Abstract

The metabolism of a C26 bile alcohol (I, 24-nor-5beta-cho-lestane-3alpha, 7alpha,25-triol) was studied in the isolated perfused rabbit liver. The new bile alcohol and bile acid metabolites secreted into the bile were isolated and identified by a combination of TLC, GLC and GLC-MS. The following bile alcohols were found: II, 24-nor-5beta-cholestane-3alpha,7alpha,12alpha,25-tetrol, III, 24-nor-5beta-cholestane-3alpha,7alpha,12alpha,25,26-pentol; IV, 24-nor-5beta-cholest-23-ene-3alpha,7alpha,12alpha-triol; and V, 24-nor-5beta-cholest-23-ene-3alpha,7alpha-diol. In the bile acid fraction, 24-nor-cholic acid and 3alpha,7alpha,12alpha-trihydroxy-24-nor-5beta-cholest-23-en-26-oic acid were present. The perfused nor-triol was not resistant to 12alpha-hydroxylation.

摘要

在离体灌注兔肝中研究了一种C26胆汁醇（I，24-去甲-5β-胆甾烷-3α，7α，25-三醇）的代谢。通过薄层层析（TLC）、气相色谱（GLC）和气相色谱-质谱联用（GLC-MS）相结合的方法，对分泌到胆汁中的新胆汁醇和胆汁酸代谢产物进行了分离和鉴定。发现了以下胆汁醇：II，24-去甲-5β-胆甾烷-3α，7α，12α，25-四醇；III，24-去甲-5β-胆甾烷-3α，7α，12α，25，26-五醇；IV，24-去甲-5β-胆甾-23-烯-3α，7α，12α-三醇；以及V，24-去甲-5β-胆甾-23-烯-3α，7α-二醇。在胆汁酸部分，存在24-去甲胆酸和3α，7α，12α-三羟基-24-去甲-5β-胆甾-23-烯-26-酸。灌注的去甲三醇对12α-羟基化不具有抗性。