Skujins J J, McLaren A D
Science. 1967 Dec 22;158(3808):1569-70. doi: 10.1126/science.158.3808.1569.
A well-mixed powder consisting of dry urea and urease exposed to air containing discrete amounts of water vapor showed a release of carbon-14 dioxide above 60-percent relative humidity. The relative activity of urease followed the water-vapor adsorption isotherm of urease. The minimum amount of water required for the reaction observed was 1.3 moles per mole of side-chain polar groups of the urease protein.
由干燥尿素和脲酶组成的充分混合的粉末,暴露于含有不同量水蒸气的空气中,在相对湿度高于60%时会释放出二氧化碳-14。脲酶的相对活性遵循脲酶的水蒸气吸附等温线。观察到的反应所需的最低水量为每摩尔脲酶蛋白质侧链极性基团1.3摩尔。