紫菜多糖的酶促降解
The enzymic degradation of porphyran.
作者信息
Turvey J R, Christison J
出版信息
Biochem J. 1967 Oct;105(1):317-21. doi: 10.1042/bj1050317.
Abstract
- The algal galactan, porphyran, was incubated with enzymes from a Cytophaga sp. and the products were examined. 2. Only about 30% of the porphyran was recovered in the form of oligosaccharides, the remainder being of high molecular weight. 3. Among the saccharides were d-galactose, 6-O-methyl-d-galactose, neoagarobiose, neoagarotetraose and oligosaccharides containing 6-O-methyl-d-galactose, the principal of which has been tentatively identified as 6(3)-O-methyl-neoagarotetraose. Fragments containing sulphate were also isolated but not identified. 4. Within the alternating sequence of the d- and l-forms of derivatives of galactose in porphyran, 6-O-methyl-d-galactose replaces d-galactose in a random manner.
摘要
- 将藻类半乳聚糖紫菜聚糖与来自噬纤维菌属的酶一起孵育，并对产物进行检测。2. 只有约30%的紫菜聚糖以低聚糖形式回收，其余为高分子量物质。3. 糖类包括d-半乳糖、6-O-甲基-d-半乳糖、新琼脂二糖、新琼脂四糖以及含有6-O-甲基-d-半乳糖的低聚糖，其中主要成分暂定为6(3)-O-甲基-新琼脂四糖。还分离出了含硫酸盐的片段，但未进行鉴定。4. 在紫菜聚糖中半乳糖衍生物的d型和l型交替序列中，6-O-甲基-d-半乳糖以随机方式取代d-半乳糖。
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