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紫菜多糖的酶促降解

The enzymic degradation of porphyran.

作者信息

Turvey J R, Christison J

出版信息

Biochem J. 1967 Oct;105(1):317-21. doi: 10.1042/bj1050317.

DOI:10.1042/bj1050317
PMID:6060448
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1198303/
Abstract
  1. The algal galactan, porphyran, was incubated with enzymes from a Cytophaga sp. and the products were examined. 2. Only about 30% of the porphyran was recovered in the form of oligosaccharides, the remainder being of high molecular weight. 3. Among the saccharides were d-galactose, 6-O-methyl-d-galactose, neoagarobiose, neoagarotetraose and oligosaccharides containing 6-O-methyl-d-galactose, the principal of which has been tentatively identified as 6(3)-O-methyl-neoagarotetraose. Fragments containing sulphate were also isolated but not identified. 4. Within the alternating sequence of the d- and l-forms of derivatives of galactose in porphyran, 6-O-methyl-d-galactose replaces d-galactose in a random manner.
摘要
  1. 将藻类半乳聚糖紫菜聚糖与来自噬纤维菌属的酶一起孵育,并对产物进行检测。2. 只有约30%的紫菜聚糖以低聚糖形式回收,其余为高分子量物质。3. 糖类包括d-半乳糖、6-O-甲基-d-半乳糖、新琼脂二糖、新琼脂四糖以及含有6-O-甲基-d-半乳糖的低聚糖,其中主要成分暂定为6(3)-O-甲基-新琼脂四糖。还分离出了含硫酸盐的片段,但未进行鉴定。4. 在紫菜聚糖中半乳糖衍生物的d型和l型交替序列中,6-O-甲基-d-半乳糖以随机方式取代d-半乳糖。

相似文献

1
The enzymic degradation of porphyran.紫菜多糖的酶促降解
Biochem J. 1967 Oct;105(1):317-21. doi: 10.1042/bj1050317.
2
The action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran.一种细菌琼脂酶对琼脂糖、紫菜聚糖和碱处理紫菜聚糖的作用。
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The hydrolysis of algal galactans by enzymes from a Cytophaga species.噬纤维菌属一种细菌产生的酶对藻类半乳聚糖的水解作用。
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引用本文的文献

1
Marine Polysaccharides: Occurrence, Enzymatic Degradation and Utilization.海洋多糖:来源、酶解与利用。
Chembiochem. 2021 Jul 1;22(13):2247-2256. doi: 10.1002/cbic.202100078. Epub 2021 May 14.
2
The hydrolysis of algal galactans by enzymes from a Cytophaga species.噬纤维菌属一种细菌产生的酶对藻类半乳聚糖的水解作用。
Biochem J. 1967 Oct;105(1):311-6. doi: 10.1042/bj1050311.
3
An extracellular agarase from a Cytophaga species.一种来自噬纤维菌属物种的胞外琼脂酶。
Biochem J. 1969 Jun;113(1):139-42. doi: 10.1042/bj1130139.
4
The extracellular agarase from a Cytophaga species.一种噬纤维菌属细菌产生的胞外琼脂酶。
Biochem J. 1968 Sep;109(2):6P. doi: 10.1042/bj1090006pa.
5
The action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran.一种细菌琼脂酶对琼脂糖、紫菜聚糖和碱处理紫菜聚糖的作用。
Biochem J. 1969 Jul;113(4):687-92. doi: 10.1042/bj1130687.
6
Isolation and characterization of Cytophaga flevensis sp. nov., a new agarolytic flexibacterium.新种弗勒沃噬纤维菌(Cytophaga flevensis sp. nov.)的分离与鉴定,一种新的琼脂分解性屈挠杆菌。
Antonie Van Leeuwenhoek. 1974;40(3):329-46. doi: 10.1007/BF00399345.
7
Shapely polysaccharides. The eighth Colworth medal lecture.形态优美的多糖。第八届科尔沃思奖章讲座。
Biochem J. 1972 Jan;126(2):257-73. doi: 10.1042/bj1260257.
8
Production and characterization of the agarase of Cytoplaga flevensis.弗氏栖胞菌琼脂酶的产生及特性研究
Antonie Van Leeuwenhoek. 1975;41(4):431-47. doi: 10.1007/BF02565087.

本文引用的文献

1
Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
2
The use of agarase from Pseudomonas atlantica in the identification of agar in marine algae (Rhodophyceae).大西洋假单胞菌琼脂酶在鉴定海藻(红藻门)中琼脂的应用。
Can J Microbiol. 1957 Dec;3(7):987-93. doi: 10.1139/m57-109.
3
The hydrolysis of algal galactans by enzymes from a Cytophaga species.噬纤维菌属一种细菌产生的酶对藻类半乳聚糖的水解作用。
Biochem J. 1967 Oct;105(1):311-6. doi: 10.1042/bj1050311.