脑苷脂的生物合成。具有不同神经节苷脂唾液酸化活性与神经节苷脂比例的膜的分离。
The biosynthesis of brain gangliosides. Separation of membranes with different ratios of ganglioside sialylating activity to gangliosides.
作者信息
Landa C A, Maccioni H J, Arce A, Caputto R
出版信息
Biochem J. 1977 Dec 15;168(3):325-32. doi: 10.1042/bj1680325c.
Brain subcellular fractions were analysed for ganglioside-sialylating activity by measuring the incorporation of N-[3H]acetylneuraminic acid from CMP-N-[3H]acetylneuraminic acid into endogenous ganglioside acceptors (endogenous incorporation) and into exogenous lactosyceramide (haematoside synthetase activity). The ratios of endogenous incorporation to gangliosides and of haematoside synthetase to gangliosides for the synaptosomal and mitochondrial fractions from a washed crude mitochondrial fraction were lower than those obtained for other membrane fractions. The differences appear to reflect intrinsic characteristics of each membrane fraction. The results of labelling in vitro and the time course of labelling of gangliosides of the different subcellular fractions in vivo after injection of N-[3H]acetylmannosamine are consistent with the possibility of a subcellular site for synthesis of gangliosides different from that of ganglioside deposition.
通过测量CMP-N-[3H]乙酰神经氨酸中N-[3H]乙酰神经氨酸掺入内源性神经节苷脂受体（内源性掺入）和外源性乳糖神经酰胺（血苷合成酶活性），分析脑亚细胞组分的神经节苷脂唾液酸化活性。来自洗涤过的粗线粒体组分的突触体和线粒体组分的神经节苷脂内源性掺入率以及血苷合成酶与神经节苷脂的比率低于其他膜组分。这些差异似乎反映了每个膜组分的内在特性。体外标记结果以及注射N-[3H]乙酰甘露糖胺后体内不同亚细胞组分神经节苷脂的标记时间进程与神经节苷脂合成的亚细胞位点不同于神经节苷脂沉积位点的可能性一致。
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