Santoro T, Stotzky G, Rem L T
Appl Microbiol. 1967 Jul;15(4):935-9. doi: 10.1128/am.15.4.935-939.1967.
Microscopic, respirometric, and electronic sizing methods for measuring germination of fungal spores were compared. With the electronic sizing method, early stages of germination (i.e., spore swelling) were detected long before germ tube emergence or significant changes in respiratory rates were observed. This method, which is rapid, easy, sensitive, and reproducible, also permits measuring the germination of spores when similar-size particles are present in concentrations considerably in excess of the number of spores.
对用于测量真菌孢子萌发的显微镜法、呼吸测定法和电子粒度分析法进行了比较。采用电子粒度分析法时,在芽管出现之前很久,或者在观察到呼吸速率有显著变化之前,就能检测到萌发的早期阶段(即孢子肿胀)。这种方法快速、简便、灵敏且可重复,当存在浓度大大超过孢子数量的类似大小的颗粒时,也能用于测量孢子的萌发。