DNA抑制因子（DSF）对同步化细胞培养物中宿主DNA合成的影响。
The effect of the DNA-suppressing factor (DSF) on host DNA synthesis in synchronized cell cultures.
作者信息
Minagawa T, Fujii N, Yamamoto T K, Iida H
出版信息
Microbiol Immunol. 1977 Nov;21(11):639-47. doi: 10.1111/j.1348-0421.1977.tb00332.x.
Abstract
Purified host DNA-suppressing factor (DSF) produced into culture fluid of HeLa C-9 cells infected with measles virus inhibited cellular DNA synthesis in HeLa cells. When purified DSF was added into cultures of synchronous HeLa cells at the early G1-phase, cellular DNA synthesis was irreversibly inhibited. However, DSF did not affect the stability of native double-stranded DNA nor the chain-elongation of single-stranded DNA in cells of the S-phase.
摘要
在感染麻疹病毒的HeLa C - 9细胞培养液中产生的纯化宿主DNA抑制因子（DSF）可抑制HeLa细胞中的细胞DNA合成。当在G1期早期将纯化的DSF添加到同步化的HeLa细胞培养物中时，细胞DNA合成被不可逆地抑制。然而，DSF并不影响S期细胞中天然双链DNA的稳定性或单链DNA的链延伸。
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