从唾液或尿液中分离出的D型逆转录病毒所致的猿猴获得性免疫缺陷综合征（SAIDS）的传播。

Transmission of simian acquired immunodeficiency syndrome (SAIDS) with type D retrovirus isolated from saliva or urine.

作者信息

Gravell M, London W T, Lecatsas G, Hamilton R S, Houff S A, Sever J L

出版信息

Proc Soc Exp Biol Med. 1984 Dec;177(3):491-4. doi: 10.3181/00379727-177-3-rc2.

Abstract

Saliva and urine specimens from rhesus monkeys with SAIDS were found to contain a type D retrovirus related to Mason-Pfizer monkey virus (MPMV) which has been linked etiologically to SAIDS. Virus isolates from saliva and urine were shown to have the characteristics of the SAIDS agent by their reverse transcriptase divalent cation preference for synthetic template-primers, production of characteristic cytopathology in Raji cells and antigenic relatedness to MPMV as determined by enzyme-linked immunosorbent assay (ELISA) and competition radioimmunoassay (RIA). Electron micrographs of parotid tissue from an animal with SAIDS also showed budding particles with type D retrovirus morphology. A tissue culture grown virus isolate from urine of an animal with SAIDS, produced SAIDS when inoculated into two normal juvenile rhesus monkeys. Since saliva and urine of monkeys with SAIDS contain infectious SAIDS virus, they are likely sources of virus by which the disease is naturally transmitted. Thus, care should be taken to avoid contact of normal and infected animals.

摘要

从患有猴获得性免疫缺陷综合征（SAIDS）的恒河猴的唾液和尿液标本中发现含有一种D型逆转录病毒，该病毒与梅森- Pfizer猴病毒（MPMV）相关，在病因上已与SAIDS联系起来。从唾液和尿液中分离出的病毒通过其逆转录酶对合成模板引物的二价阳离子偏好、在Raji细胞中产生特征性细胞病变以及通过酶联免疫吸附测定（ELISA）和竞争放射免疫测定（RIA）确定的与MPMV的抗原相关性，显示出具有SAIDS病原体的特征。一只患有SAIDS的动物的腮腺组织的电子显微镜照片也显示出具有D型逆转录病毒形态的出芽颗粒。从一只患有SAIDS的动物尿液中分离出的一种在组织培养中生长的病毒，接种到两只正常的幼年恒河猴体内时会引发SAIDS。由于患有SAIDS的猴子的唾液和尿液中含有传染性SAIDS病毒，它们很可能是该疾病自然传播的病毒来源。因此，应注意避免正常动物与感染动物接触。