Linser P J, Perkins M S, Fitch F W, Moscona A A
Biochem Biophys Res Commun. 1984 Dec 14;125(2):690-7. doi: 10.1016/0006-291x(84)90594-1.
Monoclonal antibodies (Mabs) were generated to avian carbonic anhydrase-C and characterized; their reactivity with human, murine, bovine, chicken and fish erythrocyte carbonic anhydrase-C, and with human carbonic anhydrase-B was investigated by ELISA and electroblot techniques. Reactivity of the Mabs with native and SDS-denatured carbonic anhydrase was compared. Mabs that recognize antigenic determinants shared by all the carbonic anhydrases examined were identified. The results demonstrate the potential usefulness of these particular probes for investigating various aspects of function, evolution, development and regulation of this important, but not well understood group of enzymes.
制备了针对禽碳酸酐酶-C的单克隆抗体并进行了表征；通过酶联免疫吸附测定（ELISA）和电印迹技术研究了它们与人、小鼠、牛、鸡和鱼红细胞碳酸酐酶-C以及人碳酸酐酶-B的反应性。比较了单克隆抗体与天然和十二烷基硫酸钠（SDS）变性碳酸酐酶的反应性。鉴定出了识别所有检测的碳酸酐酶共有的抗原决定簇的单克隆抗体。结果表明，这些特定探针对于研究这一重要但尚未完全了解的酶类的功能、进化、发育和调节的各个方面具有潜在用途。
