A new image analysis method for the quantification of neovascularization.

In a foregoing paper, an attempt has been described to quantify the neovascularization in the chorioallantoic membrane (CAM) by using the method of automatic image analysis. In this case 5 to 10 min were necessary to measure the neovascular response of one CAM preparation (i.e. to obtain a value for the degree of angiogenesis). The accuracy of reproducibility of the measurements was about +/- 20%. In this paper, we describe a new method which only requires about 55 seconds for the measurement of one specimen with an accuracy of about +/- 12%.