Malý A
Folia Biol (Praha). 1984;30(6):404-11.
The 60S RNA was isolated from AMV-chicken leukaemic plasma virus. It was poly(A) selected, sized on sucrose gradients, and each fraction of RNA translated in vitro in the rabbit reticulocyte lysate. It was shown by immunoprecipitation and peptide mapping that p76gag was translated from genome-length RNA, a 76K pol-related polypeptide from 24-26S RNA and a 34K protein, not related to any virion proteins, from 14S RNA. The possible relationship of these in vitro translated proteins to known genome structure will be discussed.
60S RNA是从禽成髓细胞瘤病毒-鸡白血病血浆病毒中分离出来的。它经过了聚腺苷酸化筛选，在蔗糖梯度上进行了大小分级，并且每个RNA组分都在兔网织红细胞裂解物中进行了体外翻译。通过免疫沉淀和肽图谱分析表明，p76gag是从基因组长度的RNA翻译而来，一种76K的与多聚酶相关的多肽是从24 - 26S RNA翻译而来，而一种34K的蛋白质（与任何病毒粒子蛋白均无关）是从14S RNA翻译而来。将讨论这些体外翻译的蛋白质与已知基因组结构的可能关系。
