Mariani M, Cianfriglia M, Neri P, Lafata M, Antoni G
J Immunol Methods. 1984 Dec 31;75(2):395-401. doi: 10.1016/0022-1759(84)90123-6.
The characterization of epitope specificity of a panel of 15 monoclonal antibodies against human chorionic somatomammotropin (hCS), previously prepared in our laboratory, allowed us to distinguish 4 antigenic determinants on the hCS molecule. Experiments were carried out with competition and sandwich assays. The results allowed us to divide our MAbs into 4 groups, recognizing 4 different epitopes, and to select 4 MAbs each distinguishing a different epitope in order to pursue further studies on the antigenic structure of hCS.
对我们实验室之前制备的一组15种抗人绒毛膜生长催乳素（hCS）单克隆抗体的表位特异性进行表征后，我们能够区分hCS分子上的4个抗原决定簇。通过竞争和夹心测定法进行了实验。结果使我们能够将单克隆抗体分为4组，它们识别4个不同的表位，并挑选出4种分别区分不同表位的单克隆抗体，以便对hCS的抗原结构进行进一步研究。
