大肠杆菌染色质中蛋白质的二维凝胶电泳分离。

Two-dimensional gel electrophoretic separation of the proteins present in chromatin of Escherichia coli.

作者信息

Lossius I, Sjåstad K, Haarr L, Kleppe K

出版信息

J Gen Microbiol. 1984 Dec;130(12):3153-7. doi: 10.1099/00221287-130-12-3153.

Abstract

The polypeptides present in 35S-labelled chromatin prepared from Escherichia coli cells, and polypeptides present in the DNA and RNA complexes obtained by micrococcal nuclease digestion of the chromatin, were analysed by two-dimensional non-equilibrium polyacrylamide gel electrophoresis. Three hundred and thirty-five 35S-labelled polypeptides were detected in the chromatin whereas the DNA- and RNA-containing fractions of the micrococcal nuclease digest contained 126 and 183 polypeptides respectively. The major basic low-molecular-weight polypeptides were found in the DNA-containing fractions.

摘要

对用35S标记的、从大肠杆菌细胞制备的染色质中存在的多肽，以及通过微球菌核酸酶消化染色质获得的DNA和RNA复合物中存在的多肽，进行二维非平衡聚丙烯酰胺凝胶电泳分析。在染色质中检测到335种35S标记的多肽，而微球菌核酸酶消化产物中含DNA和含RNA的部分分别含有126种和183种多肽。主要的碱性低分子量多肽存在于含DNA的部分中。