Aussedat J, Verdys M, Rossi A
Arch Int Physiol Biochim. 1984 Oct;92(3):203-17. doi: 10.3109/13813458409104501.
The rate of synthesis of myocardial adenine nucleotides from exogenous adenosine was studied in the isolated rat heart perfused under normoxic conditions and following ischaemia. The rate of incorporation of adenosine depended on the extracellular concentration of the precursor, following Michaelis-Menten kinetics with a apparent Km of 51.3 microM and a maximal rate of incorporation of about 1 100 nmol g-1 (wet wt.) 30 min-1. The adenosine uptake induced an increase in ATP concentration (+ 20%) when the exogenous concentration of precursor exceeded 10 microM. Following low-flow ischaemia (0.5 ml/min, 30 min), the rate of incorporation of 5 microM adenosine was diminished (-23%), but adenine nucleotide level restoration was favoured by the nucleoside administration. After total ischaemia (24 min), the extent of the decrease in adenosine incorporation was the same as in the case of moderate ischaemia but adenine nucleotide content was not restored.
在常氧条件下及缺血后灌注的离体大鼠心脏中，研究了外源性腺苷合成心肌腺嘌呤核苷酸的速率。腺苷的掺入速率取决于前体的细胞外浓度，遵循米氏动力学，表观Km为51.3微摩尔，最大掺入速率约为1100纳摩尔·克-1（湿重）·30分钟-1。当前体的外源性浓度超过10微摩尔时，腺苷摄取会导致ATP浓度升高（+20%）。在低流量缺血（0.5毫升/分钟，30分钟）后，5微摩尔腺苷的掺入速率降低（-23%），但核苷给药有利于腺嘌呤核苷酸水平的恢复。在完全缺血（24分钟）后，腺苷掺入减少的程度与中度缺血时相同，但腺嘌呤核苷酸含量未恢复。
