Laurent A G, Gruest J, Svab J, Montagnier L, Meurs E
Dev Biol Stand. 1984;57:305-10.
A mouse hybridoma cell line has been isolated, which secretes a monoclonal antibody (HBA) specific of human leucocyte (alpha) interferon. This monoclonal antibody neutralizes biological activities (cellular and antiviral) of most of the molecular species of alpha interferon produced by human leucocytes. Bound to Sepharose, it has been used as an immunoadsorbent (HBA-Sepharose) to purify human leucocyte interferon. Human leucocyte interferon can be purified to homogeneity by a two-step chromatography on the immunoadsorbent. A high recovery (70-90%) of purified leucocyte interferon is obtained from crude or partially purified preparations, with a specific activity of 2,8 X 10(8) IU/mg of protein. Analysis of the purified proteins by SDS-gel electrophoresis and silver staining shows that the purified human leucocyte interferon consists of three species of molecular weight: 18 000-21 000 and 27 000. This simple and rapid technique can be applied for the preparation of homogeneous leucocyte interferon on a large scale.
已分离出一种小鼠杂交瘤细胞系，它分泌一种针对人白细胞（α）干扰素的单克隆抗体（HBA）。这种单克隆抗体可中和人白细胞产生的大多数α干扰素分子种类的生物活性（细胞活性和抗病毒活性）。与琼脂糖凝胶结合后，它被用作免疫吸附剂（HBA - 琼脂糖凝胶）来纯化人白细胞干扰素。人白细胞干扰素可通过在免疫吸附剂上进行两步层析纯化至均一性。从粗制品或部分纯化制品中可获得高回收率（70 - 90%）的纯化白细胞干扰素，其比活性为2.8×10⁸IU/mg蛋白质。通过SDS - 凝胶电泳和银染对纯化蛋白质进行分析表明，纯化的人白细胞干扰素由三种分子量的分子组成：18000 - 21000和27000。这种简单快速的技术可用于大规模制备均一的白细胞干扰素。
