Greif K F, Trenchard H I
J Cell Biochem. 1984;26(2):127-33. doi: 10.1002/jcb.240260208.
The effect of neonatal deafferentation on the expression of a neuronal cell surface heparan sulfate proteoglycan (HeS-PG) was investigated in the developing rat superior cervical ganglion. Two monoclonal antibodies, one directed against the core protein of HeS-PG, and one to a determinant associated with a heparan sulfate side-chain, were used to monitor postnatal increases of HeS-PG by radioimmunoassay. Following neonatal deafferentation by section of the cervical sympathetic trunk, total protein per ganglion was slightly reduced at survival times of 7, 14, and 30 days. Expression of the core protein determinant on HeS-PG was not altered in deafferented ganglia. In contrast, levels of side-chain determinant were significantly reduced at 14 and 30 days. These results suggest that processing of HeS-PG side-chains by principal ganglionic neurons is partially regulated by transsynaptic influences during development. Transsynaptic regulation of neuronal development may be a more general process than was believed previously, with effects not limited to molecules associated with synaptic development.
在发育中的大鼠颈上神经节中，研究了新生期去传入神经对神经元细胞表面硫酸乙酰肝素蛋白聚糖（HeS-PG）表达的影响。使用两种单克隆抗体，一种针对HeS-PG的核心蛋白，另一种针对与硫酸乙酰肝素侧链相关的决定簇，通过放射免疫测定法监测出生后HeS-PG的增加。在通过切断颈交感干进行新生期去传入神经后，在存活7、14和30天时，每个神经节的总蛋白略有减少。去传入神经的神经节中HeS-PG上核心蛋白决定簇的表达没有改变。相比之下，在14天和30天时，侧链决定簇的水平显著降低。这些结果表明，在发育过程中，主要神经节神经元对HeS-PG侧链的加工受到跨突触影响的部分调节。神经元发育的跨突触调节可能是一个比以前认为的更普遍的过程，其影响不限于与突触发育相关的分子。
