Komabayashi T, Nakano K, Izawa T, Nakamura T, Tsuboi M
Jpn J Pharmacol. 1984 Dec;36(4):441-7. doi: 10.1254/jjp.36.441.
Effects of Ca2+ and calmodulin antagonists on the oxygen uptake induced by acetylcholine (ACh) or substance P (SP) were investigated in rat submandibular gland slices. The oxygen uptake induced by ACh or SP was significantly inhibited by removing Ca2+ from the medium and the slices. The oxygen uptake by ACh in the Ca2+-deficient slices was almost completely recovered by the addition of 3.0 and 5.0 mM Ca2+, whereas that by SP was not recovered by the addition of 3.0 mM Ca2+, but recovered by 5.0 mM Ca2+. Ca2+ antagonists, diltiazem, verapamil and La3+, significantly inhibited the ACh-induced oxygen uptake. On the other hand, the SP-induced oxygen uptake was inhibited by diltiazem and La3+, but not by verapamil. Calmodulin antagonists, trifluoperazine, chlorpromazine and W-7, had no inhibitory effects on the ACh-induced oxygen uptake. The SP-induced oxygen uptake was not affected by trifluoperazine, chlorpromazine and low concentrations of W-7, but was inhibited by high concentrations of W-7. These results suggest that the ACh- or SP-induced oxygen uptake is dependent on the presence and permeability of Ca2+ with a subtle difference between the ACh and the SP mechanisms and that the oxygen uptake is independent of calmodulin.
在大鼠下颌下腺切片中研究了钙离子（Ca2+）和钙调蛋白拮抗剂对乙酰胆碱（ACh）或P物质（SP）诱导的氧摄取的影响。从培养基和切片中去除Ca2+可显著抑制ACh或SP诱导的氧摄取。在缺乏Ca2+的切片中，添加3.0和5.0 mM Ca2+可使ACh诱导的氧摄取几乎完全恢复，而添加3.0 mM Ca2+不能使SP诱导的氧摄取恢复，但添加5.0 mM Ca2+可使其恢复。Ca2+拮抗剂地尔硫卓、维拉帕米和La3+显著抑制ACh诱导的氧摄取。另一方面，地尔硫卓和La3+可抑制SP诱导的氧摄取，但维拉帕米无此作用。钙调蛋白拮抗剂三氟拉嗪、氯丙嗪和W-7对ACh诱导的氧摄取无抑制作用。三氟拉嗪、氯丙嗪和低浓度的W-7对SP诱导的氧摄取无影响，但高浓度的W-7可抑制该氧摄取。这些结果表明，ACh或SP诱导的氧摄取依赖于Ca2+的存在和通透性，ACh和SP机制之间存在细微差异，且氧摄取与钙调蛋白无关。
