Wilson J E, Smith A D
Anal Biochem. 1984 Nov 15;143(1):179-87. doi: 10.1016/0003-2697(84)90574-8.
The linear polyacrylamide gradient gel electrophoresis method of Lambin and Fine (1979, Anal. Biochem. 98, 160-168) has been adapted for estimation of the molecular weights of antigen-antibody complexes, thereby providing information useful in epitope mapping studies with monoclonal antibodies. The method has been applied to mapping of the epitopes recognized by four different monoclonal antibodies raised against rat brain hexokinase (1984, Finney et al., J. Biol. Chem., 259, 8232-8237). The results obtained with the gel electrophoresis method were in agreement with epitope mapping studies conducted by the molecular sieve high-performance liquid chromatographic method of Crawford et al., (1982, Proc. Natl. Acad. Sci. USA 79, 7031-7035). Epitope mapping by the gel electrophoretic method offers several advantages in terms of speed, convenience, and economy when compared with alternative procedures that have been described.
Lambin和Fine（1979年，《分析生物化学》98卷，第160 - 168页）的线性聚丙烯酰胺梯度凝胶电泳方法已被改编用于估计抗原 - 抗体复合物的分子量，从而为单克隆抗体的表位图谱研究提供有用信息。该方法已应用于绘制针对大鼠脑己糖激酶产生的四种不同单克隆抗体所识别的表位图谱（1984年，Finney等人，《生物化学杂志》，259卷，第8232 - 8237页）。凝胶电泳方法获得的结果与Crawford等人（1982年，《美国国家科学院院刊》79卷，第7031 - 7035页）通过分子筛高效液相色谱法进行的表位图谱研究结果一致。与已描述的其他方法相比，凝胶电泳法进行表位图谱分析在速度、便利性和经济性方面具有多个优势。
