Sakamoto C, Ohki A, Otsuki M, Maeda M, Yamasaki T, Terashi K, Morita S, Baba S
Horm Metab Res. 1984 Dec;16 Suppl 1:7-10. doi: 10.1055/s-2007-1014887.
The effects of peroral treatment with synthetic trypsin inhibitor on endocrine secretion in response to four different stimuli were investigated in the isolated perfused rat pancreas. After 10 days' treatment pancreatic wet weights and protein and amylase contents in the pancreas were significantly increased. On the other hand, total contents of IRI and IRG in the T.I.-treated and control pancreas were nearly the same. In spite of the lack of alteration in hormone contents, a lower initial phase of IRI response to 8.3 mM glucose, glucose plus 0.1 ng/ml caerulein or 20 mM arginine was observed in the T.I.-treated pancreas. Furthermore, the IRI response to 10 mM theophylline was both slower and the most reduced in the T.I.-treated pancreas. From these observations, it is suggested that short-term peroral administration of synthetic trypsin inhibitor not only has no trophic effect on pancreatic B-cell and A-cell in the rat but also may affect some pathway of insulin secretion from the B-cell to reduce IRI responses to secretagogues.
在离体灌注大鼠胰腺中，研究了口服合成胰蛋白酶抑制剂对四种不同刺激下内分泌分泌的影响。经过10天的治疗，胰腺湿重以及胰腺中的蛋白质和淀粉酶含量显著增加。另一方面，经胰蛋白酶抑制剂处理的胰腺和对照胰腺中胰岛素免疫反应性（IRI）和胰高血糖素免疫反应性（IRG）的总含量几乎相同。尽管激素含量没有变化，但在经胰蛋白酶抑制剂处理的胰腺中，观察到IRI对8.3 mM葡萄糖、葡萄糖加0.1 ng/ml雨蛙素或20 mM精氨酸的反应的初始阶段较低。此外，在经胰蛋白酶抑制剂处理的胰腺中，IRI对10 mM茶碱的反应既较慢且降低最多。从这些观察结果来看，提示短期口服合成胰蛋白酶抑制剂不仅对大鼠胰腺B细胞和A细胞没有营养作用，而且可能影响B细胞胰岛素分泌的某些途径，从而降低IRI对促分泌剂的反应。
