Futaki T, Yamane M, Shirahata A, Ohta T, Hirai K
Acta Otolaryngol Suppl. 1984;419:71-8.
During endolymphatic sac surgery on patients with Meniere's disease, very small amounts (1 microliter) of the intra-sac endolymph were obtained with micro-glass pipets. An immunological assay of microanalysis of the protein by the single radial immunodiffusion (SRID) method was applied to 2 patients, one with bilateral involvement and the other unilateral. Among the protein fractions Alb, IgG, IgM, IgA, C3, C4, transferrin and alpha 2M, the concentrations of IgG were relatively higher, especially in the Meniere patient in the active stage. From 2 other patients and a control deaf patient with temporal bone fracture, the same amounts of endolymph were obtained, accompanied by a tiny specimen of the outer wall of the sac. When a radio-immunological procedure was applied to the endolymph and the homogenized sac, the specific concentration of IgG was higher in the Meniere patients than in the control.
在梅尼埃病患者的内淋巴囊手术过程中，用微型玻璃吸管获取了极少量（1微升）的囊内淋巴。采用单向辐射免疫扩散（SRID）法对蛋白质进行微量免疫分析，应用于2例患者，1例为双侧受累，另1例为单侧受累。在蛋白质组分白蛋白（Alb）、免疫球蛋白G（IgG）、免疫球蛋白M（IgM）、免疫球蛋白A（IgA）、补体C3、补体C4、转铁蛋白和α2巨球蛋白（alpha 2M）中，IgG的浓度相对较高，尤其是在活动期的梅尼埃病患者中。从另外2例患者以及1例患有颞骨骨折的对照失聪患者中，获取了相同量的内淋巴，并附带一小份囊外壁标本。当对内淋巴和匀浆后的囊进行放射免疫检测时，梅尼埃病患者中IgG的特定浓度高于对照组。
