Akaike N
Nihon Yakurigaku Zasshi. 1984 Feb;83(2):93-104.
Cl- current in GABA-sensitive neurons of the frog dorsal root ganglia was separated from other Na+, Ca2+ and K+ currents using the suction pipette technique which allows internal perfusion and current clamp. Adequacy of the internal perfusion technique was assessed from the reversal potential for GABA-induced Cl- current (EGABA) at various intracellular Cl- concentrations. EGABA was equal to the Cl- equilibrium potential (ECl) and behaved as a simple Cl- electrode following changes of external and internal Cl- concentrations [(Cl]0 and [Cl]i. EGABA changed by 58 mV for a tenfold change in either [Cl]0 or [Cl]i at constant [Cl]0 or [Cl]i, respectively. GABA-induced Cl- conductance increased dose-dependently. In addition, single Cl- channel current (icl) and conductance (gamma Cl) were estimated from the Cl- current fluctuation by using both power spectrum and amplitude histogram methods, and the theoretical values were compared with those measured directly by a conventional inside-out patch clamp technique.
利用允许内部灌注和电流钳制的吸管技术，将青蛙背根神经节中对γ-氨基丁酸（GABA）敏感的神经元中的氯离子电流与其他钠离子、钙离子和钾离子电流分离。通过在不同细胞内氯离子浓度下GABA诱导的氯离子电流（EGABA）的反转电位来评估内部灌注技术的充分性。EGABA等于氯离子平衡电位（ECl），并且在外部和内部氯离子浓度（[Cl]0和[Cl]i）变化后表现为一个简单的氯离子电极。在[Cl]0或[Cl]i恒定的情况下，当[Cl]0或[Cl]i有十倍变化时，EGABA分别变化58 mV。GABA诱导的氯离子电导呈剂量依赖性增加。此外，通过功率谱和幅度直方图方法从氯离子电流波动中估计单氯离子通道电流（icl）和电导（γCl），并将理论值与通过传统的内面向外膜片钳技术直接测量的值进行比较。
