Tsuneta H
Hokkaido Igaku Zasshi. 1984 Mar;59(2):128-39.
For thr purpose of clarifing the role of cultured thymic epithelium (CTE) and dialyzable leukocyte extracts (DLE) in the maturation process of T cells, the effects of the thymic epithelial supernatant (TES) and DLE on cyclic nucleotides in thymocytes were studied. TES increased cAMP levels significantly in thymocytes of mice. The activity of TES to increase cAMP levels correlated well with the state of the growth of thymic epithelium. Moreover, TES increased cAMP levels in human thymocytes, and augumented lymphocyte transformation (LT) to mitogens in immunodeficiency diseases. From these effects, it was suggested that TES had the activities such as thymic hormones. CTE of which TES increased cAMP levels in thymocytes of mice were transplanted in patients with Wiskott-Aldrich syndrome and Ataxia-telangiectasia. After the transplantation, augumentation of LT was observed in both patients. From these results, it was speculated that CTE were engrafted and became to exert its effect in the host. We concluded that it was possible to select the CTE appropriately for transplantation by means of examining the activity of TES. The basal levels of human thymocytes were very low compared with those of peripheral blood lymphocytes (PBL). A significant increase of cAMP levels was observed in thymocytes with stimulation of DLE. DLE produced no significant change of cyclic nucleotide levels in PBL. These results suggested that DLE affected the maturation of human thymocytes with involvement of cAMP. Though DLE was proved to contain histamin and prostaglandin E2, it was revealed from the present study that the active component responsible to increase cAMP levels in human thymocytes was different from these substances. Fractions III and IV of DLE obtained with gel filtration showed the activity to increase cAMP. It was suggested that these fractions contained the active component.
为了阐明培养的胸腺上皮细胞（CTE）和可透析白细胞提取物（DLE）在T细胞成熟过程中的作用，研究了胸腺上皮细胞上清液（TES）和DLE对胸腺细胞中环核苷酸的影响。TES显著提高了小鼠胸腺细胞中的cAMP水平。TES提高cAMP水平的活性与胸腺上皮细胞的生长状态密切相关。此外，TES提高了人胸腺细胞中的cAMP水平，并增强了免疫缺陷疾病中淋巴细胞对有丝分裂原的转化（LT）。从这些作用来看，提示TES具有胸腺激素等活性。将能使小鼠胸腺细胞中cAMP水平升高的CTE移植到Wiskott-Aldrich综合征和共济失调-毛细血管扩张症患者体内。移植后，在这两名患者中均观察到LT增强。从这些结果推测，CTE被植入并在宿主体内发挥作用。我们得出结论，通过检测TES的活性可以适当地选择用于移植的CTE。与人外周血淋巴细胞（PBL）相比，人胸腺细胞的基础水平非常低。在DLE刺激下，胸腺细胞中观察到cAMP水平显著升高。DLE对PBL中的环核苷酸水平没有产生显著变化。这些结果表明，DLE通过cAMP参与影响人胸腺细胞的成熟。尽管已证明DLE含有组胺和前列腺素E2，但本研究表明，导致人胸腺细胞中cAMP水平升高的活性成分与这些物质不同。通过凝胶过滤获得的DLE的组分III和IV显示出提高cAMP的活性。提示这些组分含有活性成分。
