Pacini D L, Dubovi E J, Clyde W A
J Infect Dis. 1984 Jul;150(1):92-7. doi: 10.1093/infdis/150.1.92.
Cotton rats (Sigmodon hispidus) were tested as a model for human respiratory tract infection due to adenovirus. After intranasal instillation of 10(6.1) 50% tissue culture infectious doses (TCID50) of adenovirus type 5 into one-month-old cotton rats, groups were killed at intervals for nasal and lung titration of virus and lung histopathology. In lung, eclipse occurred at 8 hr followed by peak viral titer (10(7.5) TCID50/g of lung) on day 5. Titers fell to 10(3.2) TCID50/g by day 10 and persisted at that level through the remainder of the study (day 28) despite appearance of serum neutralizing antibody after day 6. Interstitial pneumonia paralleled viral growth, and peribronchial mononuclear infiltration followed one to two days later. Titers in nasal mucosa peaked on day 3 but were undetectable beyond day 21. Pulmonary histopathology and viral replicative patterns paralleled findings in natural human disease.
棉鼠（棉鼠属）被用作腺病毒引起的人类呼吸道感染模型进行测试。将10(6.1) 50%组织培养感染剂量（TCID50）的5型腺病毒经鼻内滴注到1月龄棉鼠体内后，每隔一段时间处死一组动物，进行鼻腔和肺部病毒滴度测定以及肺部组织病理学检查。在肺部，8小时出现隐蔽期，随后在第5天病毒滴度达到峰值（10(7.5) TCID50/克肺组织）。到第10天，滴度降至10(3.2) TCID50/克，并且在研究的剩余时间（第28天）一直维持在该水平，尽管在第6天后出现了血清中和抗体。间质性肺炎与病毒生长平行，支气管周围单核细胞浸润在1至2天后出现。鼻黏膜中的滴度在第3天达到峰值，但在第21天后无法检测到。肺部组织病理学和病毒复制模式与人类自然疾病中的发现相似。
