脑钙调蛋白依赖性蛋白激酶对钙调蛋白敏感的环核苷酸磷酸二酯酶的磷酸化及激活作用
Phosphorylation and activation of calmodulin-sensitive cyclic nucleotide phosphodiesterase by a brain Ca2+, calmodulin-dependent protein kinase.
作者信息
Fukunaga K, Yamamoto H, Tanaka E, Iwasa T, Miyamoto E
出版信息
Life Sci. 1984 Jul 30;35(5):493-9. doi: 10.1016/0024-3205(84)90242-x.
A Ca2+, calmodulin-dependent protein kinase from rat brain with a MW of 640,000 phosphorylated calmodulin-sensitive phosphodiesterase from the brain cytosol. The Km of the enzyme for the phosphodiesterase was 5.0 microM and the Vmax was 212 nmol/mg/min. The amount of phosphate incorporated into the phosphodiesterase was 0.7 mol/mol subunit. Phosphorylation of the phosphodiesterase enhanced the enzyme activity by about 20% for hydrolysis of a higher concentration of cyclic AMP.
一种来自大鼠大脑、分子量为640,000的钙调蛋白依赖性蛋白激酶使来自脑细胞质溶胶的钙调蛋白敏感型磷酸二酯酶发生磷酸化。该酶作用于磷酸二酯酶的米氏常数为5.0微摩尔，最大反应速度为212纳摩尔/毫克/分钟。掺入磷酸二酯酶的磷酸盐量为0.7摩尔/摩尔亚基。磷酸二酯酶的磷酸化使该酶对较高浓度环磷酸腺苷水解的活性提高了约20%。
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