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硬骨鱼致癌相关重要器官的形态学研究:关注固定方法

Morphological survey of teleost organs important in carcinogenesis with attention to fixation.

作者信息

Hinton D E, Walker E R, Pinkstaff C A, Zuchelkowski E M

出版信息

Natl Cancer Inst Monogr. 1984 May;65:291-320.

PMID:6087142
Abstract

Key features of the gross and microscopic anatomy of teleost liver, digestive tract, kidney, and skin were reviewed from the world literature. Illustrations of the above and new findings were obtained from light as well as scanning and transmission electron micrographs of tissues from 7 freshwater fish species: Alosa sapidissima, American shad; Esox lucius, northern pike; Lepomis macrochirus, bluegill sunfish; Pimephales promelas, fathead minnow; Stizostedion vitreum, walleye; Oryzias latipes, Japanese medaka; and Ictalurus nebulosus, brown bullhead catfish. Emphasis was placed on structural features that may influence interpretation of toxic and carcinogenic lesions. A discussion of fixation of small fish for light and electron microscopy and histochemistry is presented.

摘要

我们从世界文献中回顾了硬骨鱼肝脏、消化道、肾脏和皮肤的大体及微观解剖学的关键特征。上述结构的插图以及新发现来自7种淡水鱼组织的光学显微镜、扫描电子显微镜和透射电子显微镜照片,这些淡水鱼分别是:美洲西鲱(Alosa sapidissima)、白斑狗鱼(Esox lucius)、蓝鳃太阳鱼(Lepomis macrochirus)、黑头软口鲦(Pimephales promelas)、大眼狮鲈(Stizostedion vitreum)、青鳉(Oryzias latipes)和褐首鲶(Ictalurus nebulosus)。重点关注了可能影响对毒性和致癌性病变解释的结构特征。本文还讨论了用于光学显微镜、电子显微镜和组织化学研究的小鱼固定方法。

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[Electron microscopic studies of the esophagus of the pike (Esox lucius L.). I. Fine structure of the "single granule cells"].[白斑狗鱼(Esox lucius L.)食管的电子显微镜研究。I. “单颗粒细胞”的精细结构]
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J Wildl Dis. 1986 Jul;22(3):435-6. doi: 10.7589/0090-3558-22.3.435.

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Isolated trout liver cells: establishing short-term primary cultures exhibiting cell-to-cell interactions.分离的鳟鱼肝细胞:建立表现出细胞间相互作用的短期原代培养物。
In Vitro Cell Dev Biol. 1990 Mar;26(3 Pt 1):237-49. doi: 10.1007/BF02624453.