Murakami K, Hashimoto K, Ota Z
Neuroendocrinology. 1984 Jul;39(1):49-53. doi: 10.1159/000123954.
Synthetic ovine corticotropin releasing factor (CRF) at 0.03-30 ng/ml evoked a significant linear release of ACTH in pituitary monolayer cell cultures. Arginine vasopressin (AVP) at 3-30 ng/ml induced a slight ACTH release in the same culture system. CRF at 100 ng/ml greatly increased the medium ACTH with intracellular ACTH remaining relatively constant. In perifused pituitary quarters, both AVP and CRF evoked significant release of ACTH. When AVP was added in combination with synthetic ovine CRF, an additive or synergistic effect was observed on the CRF-induced ACTH release in both in vitro systems. Preincubation of cultured pituitary cells with synthetic ovine CRF for 1 h did not have a significant effect on the subsequent AVP-induced ACTH release. However, when cells were preincubated for 6 h with CRF at 1 and 100 ng/ml, the AVP-induced ACTH release was significantly increased, but preincubation with AVP suppressed both the basal and AVP-induced ACTH release. These results suggest that synthetic ovine CRF stimulates not only the release of ACTH but also the synthesis of ACTH and that AVP can evoke significant ACTH release from corticotrophs which have enough releasable ACTH or substances needed for ACTH release under the influence of CRF.
在垂体单层细胞培养中，0.03 - 30纳克/毫升的合成羊促肾上腺皮质激素释放因子（CRF）引起促肾上腺皮质激素（ACTH）显著的线性释放。在相同培养系统中，3 - 30纳克/毫升的精氨酸血管加压素（AVP）诱导轻微的ACTH释放。100纳克/毫升的CRF使培养基中的ACTH大幅增加，而细胞内的ACTH保持相对恒定。在垂体组织块灌流实验中，AVP和CRF均引起ACTH的显著释放。当AVP与合成羊CRF联合添加时，在两个体外系统中均观察到对CRF诱导的ACTH释放有相加或协同作用。用合成羊CRF对培养的垂体细胞预孵育1小时，对随后AVP诱导的ACTH释放没有显著影响。然而，当细胞用1和100纳克/毫升的CRF预孵育6小时时，AVP诱导的ACTH释放显著增加，但用AVP预孵育会抑制基础ACTH释放和AVP诱导的ACTH释放。这些结果表明合成羊CRF不仅刺激ACTH的释放，还刺激ACTH的合成，并且AVP可以从在CRF影响下有足够可释放ACTH或ACTH释放所需物质的促肾上腺皮质细胞中引起显著的ACTH释放。
