Riley E P, Barron S, Driscoll C D, Chen J S
Teratology. 1984 Jun;29(3):325-31. doi: 10.1002/tera.1420290303.
The effects of prenatal alcohol exposure on the development of a conditioned taste aversion were examined in preweanling rat pups. Mothers of these pups were fed isocaloric liquid diets containing either 35 or 0% ethanol-derived calories (EDC) from gestation days 6 through 20. A pair-feeding procedure was employed, and an ad lib lab chow control group was also included. At 5, 10, or 15 days of age, pups were infused with a saccharin solution through a cannula implanted in the oral cavity. Half of the pups in each group were then injected with lithium chloride (LiCl), which served as the poisoning agent, and the other half with sodium chloride (NaCl) as a control. Animals were subsequently tested for a conditioned aversion to the saccharin solution. At 15 days of age, all of the pups in the LiCl-poisoned group demonstrated a conditioned taste aversion to the saccharin solution, but the degree of this aversion was less in alcohol-exposed offspring. At 10 days of age, a taste aversion was learned, although it was not as strong as that shown by 15-day-old pups, and it appeared to be learned equally well by all of the prenatal treatment groups. At 5 days of age, there was marginal support for taste aversion learning. Again, it did not interact with prenatal treatment. The ontogenic differences in taste aversion learning exhibited by alcohol-exposed offspring relative to controls are discussed in terms of altered hippocampal development.
在断奶前的幼鼠中研究了产前酒精暴露对条件性味觉厌恶发展的影响。这些幼鼠的母亲在妊娠第6天至第20天期间喂食等热量的液体饮食，其中一种饮食含有35%或0%的乙醇衍生热量（EDC）。采用配对喂养程序，还纳入了自由采食实验室饲料的对照组。在5、10或15日龄时，通过植入口腔的套管给幼鼠注入糖精溶液。然后，每组一半的幼鼠注射氯化锂（LiCl）作为中毒剂，另一半注射氯化钠（NaCl）作为对照。随后对动物进行测试，以检测其对糖精溶液的条件性厌恶。在15日龄时，所有注射LiCl的幼鼠都表现出对糖精溶液的条件性味觉厌恶，但酒精暴露后代的这种厌恶程度较轻。在10日龄时，幼鼠学会了味觉厌恶，尽管不如15日龄幼鼠表现得强烈，而且所有产前治疗组似乎学得同样好。在5日龄时，对味觉厌恶学习的支持很微弱。同样，它与产前治疗没有相互作用。从海马体发育改变的角度讨论了酒精暴露后代与对照组相比在味觉厌恶学习方面的个体发生差异。
