Studies of the antigenic structure of Trichophyton rubrum, Trichophyton mentagrophytes, Microsporon canis, and Epidermophyton floccosum by crossed immuno-electrophoresis.

By means of crossed immuno-electrophoresis, 35 antigens of T. rubrum, 26 of T. mentagrophytes, 35 of M. canis, and 25 of E. floccosum have been demonstrated. Tandem crossed immuno-electrophoresis has revealed two antigens common to T. rubrum and the other species, which, however, differ within the species. No common antigens have been demonstrated between T. mentagrophytes, M. canis, and E. floccosum. The technique for preparation of extracts and production of immune serum is described, and the procedure for the electrophoresis is outlined.