Eke P I, Rotimi V O, Odugbemi T O, Dosunmu-Ogunbi O O
Afr J Med Med Sci. 1984 Mar-Jun;13(1-2):15-20.
Two hundred and ninety-six bacterial isolates were investigated for the effects of saliva and alpha-amylase on their susceptibility to ampicillin, tetracycline, chloramphenicol and gentamicin. When the test organisms were primed with normal and 'diseased' saliva there were no observable differences in the MICs of ampicillin and chloramphenicol for group-A streptococci, but alpha-amylase significantly reduced the MIC of tetracycline from 2 to 0.25 mg/1. With Staphyloccus aureus, priming with saliva and alpha-amylase had no effect on the MICs of gentamicin and ampicillin, whereas the MICs of tetracycline and chloramphenicol were increased. The effect of saliva on the susceptibility of E. coli to tetracycline was also significant; MIC50 and MIC90 were reduced from 128 to 8 and 32 mg/1 respectively. Chloramphenicol was however increased from less than 0.125 to 1 and 2 mg/1 when E. coli was primed with amylase and saliva respectively. The general significance of these observations is discussed.
对296株细菌分离株研究了唾液和α-淀粉酶对其氨苄西林、四环素、氯霉素和庆大霉素敏感性的影响。当用正常唾液和“患病”唾液预处理受试微生物时，A组链球菌对氨苄西林和氯霉素的最低抑菌浓度（MIC）没有明显差异，但α-淀粉酶使四环素的MIC从2mg/1显著降至0.25mg/1。对于金黄色葡萄球菌，用唾液和α-淀粉酶预处理对庆大霉素和氨苄西林的MIC没有影响，而四环素和氯霉素的MIC增加。唾液对大肠杆菌四环素敏感性的影响也很显著；MIC50和MIC90分别从128mg/1降至8mg/1和32mg/1。然而，当分别用淀粉酶和唾液预处理大肠杆菌时，氯霉素的MIC从低于0.125mg/1增加到1mg/1和2mg/1。讨论了这些观察结果的普遍意义。
