Goldman J E, Chiu F C
Brain Res. 1984 Jul 23;306(1-2):85-95. doi: 10.1016/0006-8993(84)90358-5.
We have examined the effects of dibutyryl-cyclic AMP (dBcAMP) on the organization and expression of filamentous proteins in astroglia. The drug produced several effects on astrocytes grown in primary cultures. Cultures ceased to grow, and cells changed shape to a contracted form, displaying thin cytoplasmic processes. Cellular levels of the intermediate filament (IF) proteins, vimentin and glial fibrillary acidic protein (GFAP), and actin, insoluble in Triton X-100, were examined by polyacrylamide gel electrophoretic analysis. The cellular content of both of the IF proteins increased concurrently, approximately doubling during a 2-week course of treatment. The content of actin associated with the Triton residue decreased, however, a biochemical alteration which correlated with a loss of stress fibers in treated cells. Treatment with sodium butyrate did not change either cell shape or cytoskeletal protein content. Filament protein expression in astrocytes can, therefore, be modulated via cAMP-dependent mechanisms. The effects do not, however, appear specific for the GFAP-type of intermediate filament.
我们研究了二丁酰环磷腺苷（dBcAMP）对星形胶质细胞中丝状蛋白的组织和表达的影响。该药物对原代培养的星形胶质细胞产生了多种作用。培养物停止生长，细胞形状变为收缩形态，呈现出纤细的细胞质突起。通过聚丙烯酰胺凝胶电泳分析检测了中间丝（IF）蛋白波形蛋白和胶质纤维酸性蛋白（GFAP）以及不溶于 Triton X-100 的肌动蛋白的细胞水平。两种 IF 蛋白的细胞含量同时增加，在为期 2 周的治疗过程中大约增加了一倍。然而，与 Triton 残渣相关的肌动蛋白含量下降，这一生化改变与处理过的细胞中应力纤维的丧失相关。丁酸钠处理既未改变细胞形状也未改变细胞骨架蛋白含量。因此，星形胶质细胞中的丝状蛋白表达可通过 cAMP 依赖性机制进行调节。然而，这些作用似乎并非对 GFAP 型中间丝具有特异性。
