Broquet P, Serres-Guillaumond M, Baubichon-Cortay H, Peschard M J, Louisot P
FEBS Lett. 1984 Aug 20;174(1):43-6. doi: 10.1016/0014-5793(84)81074-1.
GDP-fucose: asialofetuin fucosyltransferase from sheep brain was fractionated on a sucrose gradient into two activity peaks. Using purification on Ficoll adapted from the proposed method [(1980) J. Neurochem. 35, 281-296], double localisation of cerebral fucosyltransferase was confirmed and the subcellular active fractions identified as light microsomes and mitochondria.
