Nagy A, Delgado-Escueta A V
J Neurochem. 1984 Oct;43(4):1114-23. doi: 10.1111/j.1471-4159.1984.tb12851.x.
A new procedure is described for the isolation of synaptosomes from various parts of mammalian brain. This method utilizes an isoosmotic Percoll/sucrose discontinuous gradient and has some advantages over the traditionally used synaptosomal isolation techniques: (1) it is possible to prepare suitable gradients while retaining isoosmolarity; (2) the time of the preparation is remarkably short (approximately 1 h); (3) if necessary, the gradient material can be easily removed from the samples. Intact synaptosomes were recovered from the 10%/16% (vol/vol) Percoll interphase. The fractions were identified and characterized by electron microscopy and by several biochemical markers for synaptosomes and other subcellular organelles. The homogeneity of the preparations is comparable to or better than that of synaptosomes prepared by the conventional methods. This procedure has been successfully used for the isolation of synaptosomes from very small tissue samples of various experimental animals and human brain.
本文描述了一种从哺乳动物脑的各个部位分离突触体的新方法。该方法利用等渗的Percoll/蔗糖不连续梯度，与传统使用的突触体分离技术相比具有一些优点：（1）能够在保持等渗的同时制备合适的梯度；（2）制备时间非常短（约1小时）；（3）如有必要，梯度材料可轻松从样品中去除。完整的突触体从10%/16%（体积/体积）的Percoll界面中回收。通过电子显微镜以及突触体和其他亚细胞器的几种生化标记物对各组分进行鉴定和表征。所制备样品的均一性与传统方法制备的突触体相当或更好。该方法已成功用于从各种实验动物和人脑的非常小的组织样品中分离突触体。
