抗利尿激素对大鼠肾小球上皮细胞中前列腺素生成的刺激作用。

Stimulation of prostaglandin production in rat glomerular epithelial cells by antidiuretic hormone.

作者信息

Lieberthal W, Levine L

出版信息

Kidney Int. 1984 May;25(5):766-70. doi: 10.1038/ki.1984.88.

DOI:10.1038/ki.1984.88

Abstract

Prostacyclin (PGI2) and prostaglandin E2 (PGE2) production by rat glomerular epithelial cells in culture were stimulated by arginine vasopressin (AVP) over a dose range of 10(-9) to 10(-6) M, but only if the cells were allowed to recover from trypsin treatment. The effect of AVP was related to its pressor activity since the antidiuretic analogue of AVP, 1-deamino-8-D-Arg-vasopressin (dDAVP) had no effect. Angiotensin II and kallidin (lysyl-bradykinin) did not affect prostaglandin production by these cells. The stimulatory effect of AVP on arachidonate metabolism was inhibited by the calcium channel antagonist, nifedipine, in a dose-dependent fashion suggesting that cellular uptake of calcium was required.

摘要

在体外培养条件下，精氨酸加压素（AVP）在10⁻⁹至10⁻⁶ M的剂量范围内可刺激大鼠肾小球上皮细胞生成前列环素（PGI2）和前列腺素E2（PGE2），但前提是细胞需从胰蛋白酶处理中恢复过来。AVP的这种作用与其升压活性相关，因为AVP的抗利尿类似物1-脱氨基-8-D-精氨酸加压素（dDAVP）无此作用。血管紧张素II和缓激肽（赖氨酰缓激肽）对这些细胞的前列腺素生成没有影响。钙通道拮抗剂硝苯地平以剂量依赖方式抑制了AVP对花生四烯酸代谢的刺激作用，这表明细胞摄取钙是必需的。