Numann R E, Wong R K
Neurosci Lett. 1984 Jun 29;47(3):289-94. doi: 10.1016/0304-3940(84)90528-7.
Acutely dissociated pyramidal cells prepared from the hippocampus of adult guinea pigs were used for the present study. The characteristic dual response of pyramidal cells to GABA as demonstrated in the in vivo and slice preparations was retained in the dissociated cells. The results show that the fading of the GABA hyperpolarizing response during prolonged GABA application cannot be accounted for by the development of an opposing depolarizing response, nor can it be explained by changes in the intracellular ionic composition during GABA action. The data strongly suggest that GABA receptors mediating the hyperpolarizing response exhibit desensitization and the time course of the GABA response is significantly controlled by this property of the receptors.
本研究使用了从成年豚鼠海马体中急性分离出的锥体神经元。锥体神经元对GABA的典型双相反应（如在体内和脑片标本中所显示的）在分离的细胞中得以保留。结果表明，在长时间应用GABA期间，GABA超极化反应的衰减既不能用相反的去极化反应的发展来解释，也不能用GABA作用期间细胞内离子组成的变化来解释。这些数据强烈表明，介导超极化反应的GABA受体表现出脱敏现象，并且GABA反应的时间进程受受体这一特性的显著控制。
