Reisman D, Sugden B
Virology. 1984 Aug;137(1):113-26. doi: 10.1016/0042-6822(84)90014-x.
A human B-lymphoid cell line, designated TG8, which does not express detectable levels of the EBV (Epstein-Barr virus)-associated nuclear antigen (EBNA), yet carries an average of one to two plasmid copies of the P3HR-1 EBV genome has been identified. TG8 can be superinfected by B95-8 EBV, resulting in up to 60-70% of the population becoming EBNA-positive and 20-30% of the incoming EBV genomes becoming circular by 48 hr postinfection. Neither EBNA expression nor the superinfecting viral DNA is maintained in the population. It is concluded that (1) superinfection of this EBV-genome-positive lymphoblast cell line leads to detectable EBNA expression and circularization of the incoming viral genome and (2) the incoming viral genome and detectable EBNA expression are selectively lost, whereas the endogenous viral plasmid DNA is maintained.
已鉴定出一种人类B淋巴细胞系，命名为TG8，它不表达可检测水平的EBV（爱泼斯坦-巴尔病毒）相关核抗原（EBNA），但平均携带1至2个P3HR-1 EBV基因组的质粒拷贝。TG8可被B95-8 EBV超感染，导致感染后48小时，多达60%-70%的细胞群体EBNA呈阳性，20%-30%的传入EBV基因组环化。在细胞群体中，EBNA表达和超感染的病毒DNA均未维持。结论是：（1）这种EBV基因组阳性淋巴母细胞系的超感染导致可检测到的EBNA表达和传入病毒基因组的环化；（2）传入病毒基因组和可检测到的EBNA表达被选择性丢失，而内源性病毒质粒DNA得以维持。
