Rottem S, Shirvan M H, Gross Z
Yale J Biol Med. 1983 Sep-Dec;56(5-6):405-11.
The osmotic stability of M. gallisepticum was found to be a consequence of the synthesis of disaturated phosphatidylcholine incorporated into the cell membrane. The disaturated lipid induces the formation of segregated lipid domains, thus providing the sites for increased permeation of ions. Such permeation reduces the internal pressure so as to minimize cell swelling and subsequent lysis in a hypotonic medium. Purified membranes of M. gallisepticum can be prepared from cells suspended in an iso-osmotic NaCl solution containing either dicyclohexylcarbodiimide (DCCD), which blocks ATPase activity, or a mild alkaline buffer. Both conditions seem to interfere with cell volume regulation. These procedures can be used also to isolate membranes of other osmotically stable mycoplasmas.
鸡毒支原体的渗透稳定性被发现是细胞膜中掺入的二饱和磷脂酰胆碱合成的结果。二饱和脂质诱导形成分离的脂质结构域，从而为离子渗透增加提供位点。这种渗透降低了内部压力，以便在低渗介质中使细胞肿胀和随后的裂解最小化。鸡毒支原体的纯化膜可以从悬浮在含有二环己基碳二亚胺（DCCD，它会阻断ATP酶活性）的等渗NaCl溶液或温和碱性缓冲液中的细胞制备。这两种条件似乎都会干扰细胞体积调节。这些方法也可用于分离其他具有渗透稳定性的支原体的膜。
