Rettori V, Van Sickle M, Steinberger A, Asch R H
Acta Eur Fertil. 1984 Mar-Apr;15(2):93-7.
Ovarian cystic fluid was obtained from 13 regularly cycling women with the operative diagnosis of ovarian serous (n = 6), mucinous cystadenomas (n = 3) and simple follicular cysts (n = 4). The effects of the fluids, after steroid extraction with charcoal treatment, on the binding of follicle stimulating hormone (FSH) to rat testicular homogenates and on the in vitro secretion of FSH and luteinizing hormone (LH) by rat pituitary cell preparations were studied. Similar studies were conducted simultaneously with steroid-free porcine follicular fluid (pFF). Human ovarian cystic fluids produced a marked inhibition of FSH-binding activity in the absence of an effect on FSH or LH secretion. In contrast, pFF exhibited both FSH-binding inhibitory (FSH-BI) and inhibin activities. Furthermore, heating did not alter the FSH-BI activity nor the inhibin properties of steroid-free human ovarian cystic fluids nor porcine follicular fluid.
从13名月经周期正常的女性身上获取了卵巢囊液，这些女性经手术诊断患有卵巢浆液性囊肿（n = 6）、黏液性囊腺瘤（n = 3）和单纯性滤泡囊肿（n = 4）。在用活性炭处理提取类固醇后，研究了这些囊液对促卵泡激素（FSH）与大鼠睾丸匀浆结合的影响，以及对大鼠垂体细胞制剂体外分泌FSH和促黄体生成素（LH）的影响。同时用无类固醇的猪卵泡液（pFF）进行了类似研究。人卵巢囊液在对FSH或LH分泌无影响的情况下，对FSH结合活性产生了显著抑制。相比之下，pFF既表现出FSH结合抑制（FSH-BI）活性，又表现出抑制素活性。此外，加热不会改变无类固醇的人卵巢囊液或猪卵泡液的FSH-BI活性及抑制素特性。
