Harrabi S, Revol A, Biot N, Perrin-Fayolle M
Ann Biol Clin (Paris). 1984;42(2):123-8.
The authors studied the parameters of Friedland and Silverstein's technique for measuring the activity of the angiotensin I conversion enzyme and found a number of anomalies. The technique was modified by correcting the wide pH difference between the pH of the reagent and the pH of the reference solution. This modification made the technique optimal for the measurement of the enzyme activity by allowing a 20 to 40 per cent increase in this activity and a 15% increase in the positivity in cases of clinical sarcoidosis. The application of this modified test to the determination of the activity of the angiotension I conversion enzyme in control subjects, patients with sarcoidosis and patients with order respiratory diseases gave the following mean values, respectively: 39 +/- 10 IU; 81 +/- 23 IU; 41.5 +/- 2.5 IU in the serum and 0.2 IU; 1.1 +/- 0.5 IU; 0.2 +/- 0.1 IU in bronchoalveolar lavage fluid. The increase in enzyme activity in sarcoidosis is found in the serum in 75% of cases and in bronchoalveolar lavage fluid in 85% of cases. This modified technique can be applied in routine practice and in research.
作者研究了弗里德兰德和西尔弗斯坦测量血管紧张素I转换酶活性的技术参数，发现了一些异常情况。通过校正试剂pH值与参考溶液pH值之间较大的差异对该技术进行了改进。这种改进使该技术在测量酶活性方面达到最佳状态，酶活性提高了20%至40%，在临床结节病病例中阳性率提高了15%。将这种改良试验应用于对照组、结节病患者和其他呼吸系统疾病患者血管紧张素I转换酶活性的测定，分别得到以下平均值：血清中为39±10 IU；81±23 IU；41.5±2.5 IU，支气管肺泡灌洗液中为0.2 IU；1.1±0.5 IU；0.2±0.1 IU。在结节病中，75%的病例血清中酶活性增加，85%的病例支气管肺泡灌洗液中酶活性增加。这种改良技术可应用于常规实践和研究。
