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中枢神经系统髓磷脂的光亲和标记。内源性I型环磷酸腺苷依赖性激酶磷酸化2',3'-环核苷酸3'-磷酸二酯酶大亚基的证据。

Photoaffinity labelling of central-nervous-system myelin. Evidence for an endogenous type I cyclic AMP-dependent kinase phosphorylating the larger subunit of 2',3'-cyclic nucleotide 3'-phosphodiesterase.

作者信息

Bradbury J M, Thompson R J

出版信息

Biochem J. 1984 Jul 15;221(2):361-8. doi: 10.1042/bj2210361.

DOI:10.1042/bj2210361
PMID:6089737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1144047/
Abstract

Endogenous cyclic AMP-stimulated phosphorylation of a 49700-Mr Wolfgram protein component in rabbit central nervous system was investigated by using photoaffinity labelling and 2',3'-cyclic nucleotide 3'-phosphodiesterase activity staining after electroblotting on to nitrocellulose paper. Photoaffinity labelling with 8'-azidoadenosine 3',5'-cyclic monophosphate showed a cyclic AMP-binding protein that appeared to be intrinsic to the myelin membrane and appeared to represent the R-subunit of a type I cyclic AMP-dependent protein kinase. This photoaffinity-labelled protein was of larger apparent Mr than the protein showing cyclic AMP-stimulated phosphorylation. Blotting of one-dimensional sodium dodecyl sulphate/polyacrylamide-gel electrophoretograms followed by staining for 2',3'-cyclic nucleotide 3'-phosphodiesterase activity showed two activity bands corresponding to the two components of the Wolfgram protein doublet. Cyclic AMP-stimulated protein phosphorylation corresponded to the upper component of this doublet. Electroblotting of two-dimensional non-equilibrium pH-gradient electrophoretograms also showed co-migration of cyclic AMP-stimulated protein phosphorylation with enzyme activity. It is proposed that central-nervous-system myelin contains an endogenous type I cyclic-AMP dependent protein kinase that phosphorylates the larger subunit of 2',3'-cyclic nucleotide 3'-phosphodiesterase.

摘要

通过光亲和标记以及在硝酸纤维素纸上进行电印迹后的2',3'-环核苷酸3'-磷酸二酯酶活性染色，研究了家兔中枢神经系统中内源性环磷酸腺苷（cAMP）刺激的49700道尔顿沃尔夫格兰姆蛋白成分的磷酸化作用。用8'-叠氮腺苷3',5'-环一磷酸进行光亲和标记显示出一种环磷酸腺苷结合蛋白，它似乎是髓鞘膜固有的，并且似乎代表I型环磷酸腺苷依赖性蛋白激酶的R亚基。这种光亲和标记的蛋白表观分子量比显示环磷酸腺苷刺激的磷酸化作用的蛋白更大。一维十二烷基硫酸钠/聚丙烯酰胺凝胶电泳图谱进行印迹，随后进行2',3'-环核苷酸3'-磷酸二酯酶活性染色，显示出两条活性带，对应于沃尔夫格兰姆蛋白双峰的两个成分。环磷酸腺苷刺激的蛋白磷酸化作用对应于该双峰的上部成分。二维非平衡pH梯度电泳图谱的电印迹也显示环磷酸腺苷刺激的蛋白磷酸化作用与酶活性共同迁移。有人提出，中枢神经系统髓鞘含有一种内源性I型环磷酸腺苷依赖性蛋白激酶，它使2',3'-环核苷酸3'-磷酸二酯酶的较大亚基磷酸化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/5d4f8b374421/biochemj00323-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/587d11fc96d6/biochemj00323-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/75b457b313ae/biochemj00323-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/07ada6e6a0fd/biochemj00323-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/669ba04ea8b0/biochemj00323-0090-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/5d4f8b374421/biochemj00323-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/587d11fc96d6/biochemj00323-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/75b457b313ae/biochemj00323-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/07ada6e6a0fd/biochemj00323-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/669ba04ea8b0/biochemj00323-0090-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb83/1144047/5d4f8b374421/biochemj00323-0091-a.jpg

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations.大鼠脑匀浆和线粒体制剂中糖酵解酶及其他酶的细胞内分布。
Biochem J. 1960 Dec;77(3):610-8. doi: 10.1042/bj0770610.
3
A new and rapid colorimetric determination of acetylcholinesterase activity.一种新的快速比色法测定乙酰胆碱酯酶活性。
2',3'-环核苷酸3'-磷酸水解酶（CNP）的体内磷酸化：脑髓鞘中的CNP可被福斯高林和佛波酯敏感的蛋白激酶磷酸化。
Neurochem Res. 1994 Jun;19(6):721-8. doi: 10.1007/BF00967712.
4
Topology of Wolfgram proteins and 2', 3'-cyclic nucleotide 3'-phosphodiesterase in CNS myelin: studies with proteases.中枢神经系统髓鞘中沃尔夫格兰姆蛋白和2',3'-环核苷酸3'-磷酸二酯酶的拓扑结构：蛋白酶研究
Neurochem Res. 1994 Sep;19(9):1113-8. doi: 10.1007/BF00965143.
5
Immunoblotting and dot blotting.免疫印迹法和斑点印迹法。
J Immunol Methods. 1989 May 12;119(2):153-87. doi: 10.1016/0022-1759(89)90394-3.
Biochem Pharmacol. 1961 Jul;7:88-95. doi: 10.1016/0006-2952(61)90145-9.
4
Phosphorylation in vivo of four basic proteins of rat brain myelin.大鼠脑髓鞘四种碱性蛋白的体内磷酸化作用
Biochem J. 1982 Jan 1;201(1):39-47. doi: 10.1042/bj2010039.
5
Regulatory mechanisms in the control of protein kinases.蛋白质激酶调控中的调节机制。
CRC Crit Rev Biochem. 1982 Feb;12(2):133-86. doi: 10.3109/10409238209108705.
6
PGP 9.5--a new marker for vertebrate neurons and neuroendocrine cells.PGP 9.5——一种脊椎动物神经元和神经内分泌细胞的新标志物。
Brain Res. 1983 Nov 14;278(1-2):224-8. doi: 10.1016/0006-8993(83)90241-x.
7
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FEBS Lett. 1982 Jul 19;144(1):77-80. doi: 10.1016/0014-5793(82)80572-3.
8
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Biochem J. 1981 Mar 1;193(3):875-85. doi: 10.1042/bj1930875.
9
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Anal Biochem. 1981 Apr;112(2):195-203. doi: 10.1016/0003-2697(81)90281-5.
10
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.从十二烷基硫酸钠 - 聚丙烯酰胺凝胶中洗脱蛋白质、去除十二烷基硫酸钠以及酶活性的复性：大肠杆菌RNA聚合酶σ亚基、小麦胚芽DNA拓扑异构酶及其他酶的实验结果
Anal Biochem. 1980 Nov 15;109(1):76-86. doi: 10.1016/0003-2697(80)90013-5.