使用铜（I）-硫脲作为铜硫蛋白模型将铜（I）转移至脱辅基星蓝蛋白中。
Copper(I) transfer into apo-stellacyanin using copper(I)-thiourea as a copper-thionein model.
作者信息
Morpurgo L, Rotilio G, Hartmann H J, Weser U
出版信息
Biochem J. 1984 Aug 1;221(3):923-5. doi: 10.1042/bj2210923.
Abstract
The direct incorporation of Cu(I) from [Cu(I)(thiourea)3]Cl, a structural analogue of Cu-thionein, into apo-stellacyanin, was successful both aerobically and anaerobically. A characteristic c.d. band of Cu(I)-stellacyanin at 270 nm (0 = -12.5 X 10(3) degrees X cm2 X dmol-1) was seen. On oxidation with hexacyanoferrate(III) or by air, the correct Cu(II) binding into the active centre of this 'Type 1' Cu-protein was deduced from chiroptical measurements which were supported by e.p.r. data. Thus Cu-thiourea turned out to be an excellent Cu(I)-donor in aqueous systems for the complete reconstitution of mononuclear Blue copper proteins.
摘要
将铜硫蛋白的结构类似物[Cu(I)(硫脲)3]Cl中的Cu(I)直接掺入脱辅基星蓝蛋白，无论在有氧还是无氧条件下均获成功。观察到Cu(I)-星蓝蛋白在270 nm处有一特征性圆二色带（θ = -12.5×10³度·厘米²·摩尔⁻¹）。在用铁氰化铁(III)或空气氧化时，通过圆二色光谱测量推断出正确的Cu(II)结合到这种“1型”铜蛋白的活性中心，电子顺磁共振数据也支持这一结果。因此，在水性体系中，铜硫脲被证明是用于单核蓝铜蛋白完全重构的优良Cu(I)供体。
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