1,25-二羟维生素D可使单核细胞样细胞中环磷酸腺苷反应减弱。
1,25 Dihydroxyvitamin D causes attenuation of cyclic AMP responses in monocyte-like cells.
作者信息
Rubin J E, Catherwood B D
出版信息
Biochem Biophys Res Commun. 1984 Aug 30;123(1):210-5. doi: 10.1016/0006-291x(84)90400-5.
The U937 cell is a human monocyte-like line which possesses 1,25(OH)2D receptors. To study 1,25(OH)2D actions in these cells we have measured the cAMP produced by U937 cells during 10 minute stimulus by the beta-adrenergic agonist isoproterenol or by forskolin. cAMP produced by isoproterenol is 6 times that of forskolin. When cells are exposed to 1,25(OH)2D for at least 8 hours the cAMP produced is decreased up to 55%. This attenuative effect of 1,25(OH)2D is dose dependent with an EC50 of 10(-10)M. Other vitamin D metabolites are less than 1/100th as potent.
U937细胞是一种具有1,25(OH)2D受体的人单核细胞样细胞系。为了研究1,25(OH)2D在这些细胞中的作用，我们测量了U937细胞在β-肾上腺素能激动剂异丙肾上腺素或福斯高林刺激10分钟期间产生的环磷酸腺苷（cAMP）。异丙肾上腺素产生的cAMP是福斯高林的6倍。当细胞暴露于1,25(OH)2D至少8小时时，产生的cAMP最多可降低55%。1,25(OH)2D的这种衰减作用具有剂量依赖性，半数有效浓度（EC50）为10(-10)M。其他维生素D代谢物的效力不到其1/100。
Zotero 插件