Stern P, Kagen L J
Exp Cell Res. 1984 Oct;154(2):485-91. doi: 10.1016/0014-4827(84)90172-1.
Growth, protein synthesis and expression of creatine kinase (CK) by embryonic chick myogenic cells are inhibited by vitamin D and certain of its metabolites. 25-OH cholecalciferol was most active in concentrations of 10(-5) - 10(-6) M, with cholecalciferol and ergocalciferol less active in that order. Ergosterol had no activity of this sort. Inhibition of CK was most marked on the 4th and 5th day of culture and was due to suppression of the appearance of CK-NM and MB. CK-BB was not affected and CK-MB was more affected than CK-BB. Skin fibroblasts by comparison were slightly stimulated in growth at 10(-6) M and much less affected at 10(-5) M than the myogenic cells. It is suggested that vitamin D has a direct effect upon the muscle cell, to cause a selective diminution in the production of certain polypeptides.
维生素D及其某些代谢产物可抑制胚胎鸡成肌细胞的生长、蛋白质合成以及肌酸激酶（CK）的表达。25-羟基胆钙化醇在浓度为10⁻⁵ - 10⁻⁶ M时活性最强，胆钙化醇和麦角钙化醇的活性依次降低。麦角固醇无此类活性。培养第4天和第5天时，CK的抑制最为明显，这是由于CK-NM和MB的出现受到抑制。CK-BB未受影响，且CK-MB比CK-BB受影响更大。相比之下，皮肤成纤维细胞在10⁻⁶ M时生长略有刺激，在10⁻⁵ M时受影响程度远小于成肌细胞。提示维生素D对肌肉细胞有直接作用，导致某些多肽的产生选择性减少。
